Journal: Comparative Immunology, Microbiology and Infectious Diseases
Article Title: Histopathological and immunohistochemical findings in lymphoid tissues of the endangered Iberian lynx ( Lynx pardinus )
doi: 10.1016/j.cimid.2006.01.003
Figure Lengend Snippet: Immunohistochemistry of CD3, CD79, MAC387 and CD68. Columns indicate the percentage of lymphoid tissues with immunostained subpopulations. LN, lymph node; MALT, mucosa associated lymphoid tissue.
Article Snippet: The primary antibodies used were: rabbit polyclonal antibody anti-CD3, pan-T cell marker (DAKO A452, dilution 1/100, incubation overnight 4 °C); mouse monoclonal antibody anti-CD79, mature B cells marker (clone HM57, DAKO M7051, dilution 1/10, incubation overnight 4 °C); mouse monoclonal antibody clone MAC 387 myeloid/histiocyte antigen (DAKO M0747, dilution 1/200, incubation overnight 4 °C); and mouse monoclonal antibody anti-CD68 macrophage (clone PG-M1) (DAKO M0876, dilution 1/20, incubation overnight 4 °C).
Techniques: Immunohistochemistry